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Ellenberger and co-personnel (8) had intuited exactly such a motion on the OB domain when they solved the crystal framework in the much bigger human DNA ligase I (HuLig1; 919 amino acids) certain to an adenylylated nick. Their landmark framework was the initial to document the circumferential envelopment of your DNA duplex by a ligase clamp and also the ensuing bending and distortion in the duplex that power the base pairs about the 3′-OH facet in the nick into an RNA-like A helical conformation (8). Comparable distortions have been noticed from the ChVLig·DNA complex (seven).
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